Munich Multiscale Biofabrication Network

Beckmann Lab

https://www.genzentrum.uni-muenchen.de/research-groups/beckmann/index.html

Structure of the yeast CAT tailing machinery on the 60S ribosomal subunit (Tesina et al., 2023).


Roland Beckmann’s lab is interested in the decoding of the molecular mechanisms underlying protein biosynthesis, its regulation and ribosome-associated activities (Fig. 1), and of eukaryotic ribosome biogenesis (Fig. 2). The central technology used is cryo-electron microscopy (cryo-EM) to determine the structure of functionally defined complexes involved in these processes. In addition, complementary molecular biological and biochemical methods are used, in particular so-called cell-free systems, to further characterize the functioning of these complexes. A long-term goal is to use these findings as a basis to intervene in or repurpose both, protein production and ribosome biogenesis, in order to develop novel macromolecules and nanomachines. A current focus is the reconstitution, analysis and manipulation of the so-called CAT tailing system (Fig. 3) which allows for 40S-, mRNA- and GTPase-independent peptide elongation during ribosomal quality control (RQC).

 

 

 

 

References

  1. P. Tesina*, S. Ebine*, R. Buschauer*, M. Thoms, Y. Matsuo, T. Inada, R. Beckmann, Molecular Cell 2023, accepted
  2. M. Ameismeier, I. Zemp, J. van den Heuvel, M. Thoms, O. Berninghausen, U. Kutay, R. Beckmann, Structural basis for the final steps of human 40S ribosome maturation. Nature 2020 , doi: 10.1038/s41586-020-2929-x
  3. J. Cheng, B. Lau, G. La Venuta, M. Ameismeier, O. Berninghausen, E. Hurt, R. Beckmann, 90S pre-ribosome transformation into the primordial 40S subunit. Science 2020 , 369(6510):1470-1476, doi: 10.1126/science.abb4119
  4. M. Thoms, R. Buschauer, M. Ameismeier, L. Koepke, T. Denk, M. Hirschenberger, H. Kratzat, M. Hayn, T. Mackens-Kiani, J. Cheng, J. H. Straub, C. M. Stürzel, T. Fröhlich, O. Berninghausen, T. Becker, F. Kirchhoff, K. Sparrer, R. Beckmann, Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. Science 2020, doi: 10.1126/science.abc8665
  5. R. Buschauer, Y. Matsuo, T. Sugiyama, Y. H. Chen, N. Alhusaini, T. Sweet, K. Ikeuchi, J. Cheng, Y. Matsuki, R. Nobuta, A. Gilmozzi, O. Berninghausen, P. Tesina, T. Becker, J. Coller, T. Inada, R. Beckmann, The Ccr4-Not complex monitors the translating ribosome for codon optimality. Science 2020 , 368(6488) pii: eaay6912, doi: 10.1126/science.aay6912
  6. T. Su, T. Izawa, M. Thoms, Y. Yamashita, J. Cheng, O. Berninghausen, F. U. Hartl, T. Inada, W. Neupert, R. Beckmann, Structure and function of Vms1 and Arb1 in RQC and mitochondrial proteome homeostasis. Nature 2019 , doi: 10.1038/s41586-019-1307-z